cellular and subcelular co-localization analysis in light microscopy

Curr Protoc Cell Biol. 2008 Jun;Chapter 4:Unit 4.19.

Quantitative colocalization analysis of confocal fluorescence microscopy images.

Department of Anatomy and Cell Biology, Kochi University, Faculty of Medicine, Japan.

Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a number of specialized coefficients. First, images of double-stained sections are subjected to background correction. Then, various coefficients are calculated. Meanings of the coefficients and a guide to interpretation of their results indicating either presence or absence of colocalization are given. Success in colocalization studies depends on the quality of analyzed images, proper preparation of them for coefficients calculations, and correct interpretation of obtained results. This protocol helps to ensure reliability of colocalization coefficients calculations. Copyright 2008 by John Wiley & Sons, Inc.

Zinchuk V, Zinchuk O.

Abstract

Quantitative colocalization analysis of confocal fluorescence microscopy images.
Curr Protoc Cell Biol. 2008 Jun;Chapter 4:Unit 4.19.
PMID: 18551422 [PubMed – in process]
2:

Comeau JW, Kolin DL, Wiseman PW.

Abstract

Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy.
Mol Biosyst. 2008 Jun;4(6):672-85. Epub 2008 Apr 16.
PMID: 18493666 [PubMed – in process]
3:
Abstract

Immunohistochemical localization of proteins in the nervous system.
Curr Protoc Neurosci. 2004 Feb;Chapter 1:Unit 1.2.
PMID: 18428596 [PubMed – indexed for MEDLINE]
4:

Scriven DR, Lynch RM, Moore ED.

Abstract

Image acquisition for colocalization using optical microscopy.
Am J Physiol Cell Physiol. 2008 May;294(5):C1119-22. Epub 2008 Mar 19.
PMID: 18353895 [PubMed – in process]
5:

Das R, Hammond S, Holowka D, Baird B.

Abstract

Real-time cross-correlation image analysis of early events in IgE receptor signaling.
Biophys J. 2008 Jun;94(12):4996-5008. Epub 2008 Mar 7.
PMID: 18326662 [PubMed – indexed for MEDLINE]
6:

Kandela IK, Bleher R, Albrecht RM.

Abstract

Immunolabeling for correlative light and electron microscopy on ultrathin cryosections.
Microsc Microanal. 2008 Apr;14(2):159-65. Epub 2008 Mar 3.
PMID: 18312726 [PubMed – indexed for MEDLINE]
7:

Reissig CJ, Rabin RA, Winter JC, Dlugos CA.

Abstract

d-LSD-induced c-Fos expression occurs in a population of oligodendrocytes in rat prefrontal cortex.
Eur J Pharmacol. 2008 Mar 31;583(1):40-7. Epub 2008 Jan 7.
PMID: 18262520 [PubMed – indexed for MEDLINE]
8:

Shigematsu N, Yamamoto K, Higuchi S, Fukuda T.

Abstract

An immunohistochemical study on a unique colocalization relationship between substance P and GABA in the central nucleus of amygdala.
Brain Res. 2008 Mar 10;1198:55-67. Epub 2008 Jan 3.
PMID: 18243164 [PubMed – indexed for MEDLINE]
9:

Nohe A, Petersen NO.

Abstract

Image correlation spectroscopy.
Sci STKE. 2007 Dec 18;2007(417):pl7.
PMID: 18089858 [PubMed – indexed for MEDLINE]
10:

Bacia K, Schwille P.

Abstract

Practical guidelines for dual-color fluorescence cross-correlation spectroscopy.
Nat Protoc. 2007;2(11):2842-56.
PMID: 18007619 [PubMed – indexed for MEDLINE]

—article of Suzanne, Gif:

Bolte S., and Cordelieres F. (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Microsc. 2006 Dec;224(Pt 3):213-32.

0 Responses to “cellular and subcelular co-localization analysis in light microscopy”



  1. Laisser un commentaire

Laisser un commentaire

Entrez vos coordonnées ci-dessous ou cliquez sur une icône pour vous connecter:

Logo WordPress.com

Vous commentez à l'aide de votre compte WordPress.com. Déconnexion / Changer )

Image Twitter

Vous commentez à l'aide de votre compte Twitter. Déconnexion / Changer )

Photo Facebook

Vous commentez à l'aide de votre compte Facebook. Déconnexion / Changer )

Photo Google+

Vous commentez à l'aide de votre compte Google+. Déconnexion / Changer )

Connexion à %s




I MOVED THIS BLOG FROM WORDPRESS TO BLOGGER. Ce blog est à
ex-ample.blogspot.com

Blog Stats

  • 206,717 hits

localization

Flickr Photos

A bellezza di a natura (C☺rsica)

Plus de photos
juillet 2008
L M M J V S D
« Juin   Août »
 123456
78910111213
14151617181920
21222324252627
28293031  

%d blogueurs aiment cette page :